Posted: December 30th, 2021
2. Using standard cloning techniques, you have obtained a genomic actin clone from chickenusing an actin probe from quail. When you use your chicken genomic actin clone against totalcellular RNA derived from a sample of skeletal muscle of chicken (Northern blot) you find that2 different RNAs show strong hybridization to the chicken actin probe. After an extensive andexhaustive search against your chicken genomic library you are unable to obtain a secondchicken genomic actin clone. The conclusion you reach is that the chicken genomic actin cloneused as probe is hybridizing to two different sized mRNAs which represent differentiallyspliced variants of a single genomic actin gene. You clone the two cDNAs which represent thetwo mRNAs and after sequence comparisons with each other and with the genomic clone youdetermine they are in fact two splice variants of a single gene. Your next experiment is toquantitatively determine the relative amounts of the two mRNAs in samples obtained fromskeletal muscle at different stages of development. Briefly outline in point form two differentexperimental procedure(s) you could perform in the lab to accomplish your goal. (8 marks)
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